Mathematical Modeling of Perifusion Cell Culture Experiments
نویسندگان
چکیده
Mathematical Modeling of Perifusion Cell Culture Experiments by Nihal Ezgi Temamogullari Department of Mathematics Duke University
منابع مشابه
Mathematical modeling of perifusion cell culture experiments on GnRH signaling.
The effects of pulsatile GnRH stimulation on anterior pituitary cells are studied using perifusion cell cultures, where constantly moving culture medium over the immobilized cells allows intermittent GnRH delivery. The LH content of the outgoing medium serves as a readout of the GnRH signaling pathway activation in the cells. The challenge lies in relating the LH content of the medium leaving t...
متن کاملEffects of zeranol on in vitro growth hormone release by lamb and rat pituitary cells.
A series of experiments was conducted to evaluate the effect of zeranol on release and synthesis of growth hormone (GH) by anterior pituitary cells established in either static or continuous flow cultures. Young adult male rats, slaughter-age lambs and juvenile lambs were used as sources of pituitary cells. In static primary cell cultures, no consistent effect of zeranol at 10(-7), 10(-9) or 10...
متن کاملA Model of Dispersion in Perifusion Systems
The perifusion apparatus is an experimental tool used to model information transfer in endocrine systems. The major drawback of the penfusion system derives from the dispersion, diffusion and mixing of the hormone within the apparatus which distort the original released hormone concentration profile. In this paper we develop a mathematical model of the perifusion system that accounts for a numb...
متن کاملNaltrexone Stimulates Lh Secretion in Vitro from the Common Carp (cyprinus Carpio L.) Pituitary Gland
The effects of naltrexone on the in vitro luteinizing hormone (LH) secretion from whole pituitary glands as well as from dispersed pituitary cells of common carp (Cyprinus carpio L.) in primary cell culture were studied. The perifusion of whole pituitary glands with naltrexone at a concentration of 10 M caused a significant increase of LH secretion compared to the control. This finding suggests...
متن کاملA review of mathematical models of influenza A infections within a host or cell culture: lessons learned and challenges ahead
Most mathematical models used to study the dynamics of influenza A have thus far focused on the between-host population level, with the aim to inform public health decisions regarding issues such as drug and social distancing intervention strategies, antiviral stockpiling or vaccine distribution. Here, we investigate mathematical modeling of influenza infection spread at a different scale; name...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016